Engineered DNA ligases with improved activities in vitro
نویسندگان
چکیده
منابع مشابه
Engineered DNA ligases with improved activities in vitro.
The DNA ligase from bacteriophage T4 is one of the most widely used enzymes in molecular biology. It has evolved to seal single-stranded nicks in double-stranded DNA, but not to join double-stranded fragments with cohesive or blunt ends. Its poor activity in vitro, particularly with blunt-ended substrates, can lead to failed or sub-optimal experimental outcomes. We have fused T4 DNA ligase to s...
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DNA ligases are Mg++-dependent enzymes that catalyze the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA (for review and main references up to 1992, see Engler and Richardson 1982; Lindahl and Barnes 1992). The first step in the reaction is the formation of a covalent enzyme/adenylate intermediate. DNA ligases from eukaryotes, archaea, and viruses employ ATP as ...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2013
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzt024